All raw data is available via the European Nucleotide Archive (ENA) under the accession code PRJEB18739.
Sample | Details | Platform | Type | Download (ENA) |
---|---|---|---|---|
WT-DSMZ | DSM 2343 (ATCC 621H), wild type for DSMZ | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769945 |
WT-BM | DSM 2343 (ATCC 621H), wild type | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769946 |
WT-E | DSM 2343 (ATCC 621H), wild type | Illumina MiSeq | 2x250bp paired-end TruSeq PCR-free | ERR1769947 |
∆upp | WT-E derivative with a deletion of upp (GOX0327 / GOX_RS02795) coding for uracil phosphoribosyl-transferase | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769948 |
IK001 | ΔuppΔgdhS::sdhCDABE - Δupp derivative with genomically integrated sdhCDAB (APA01_00310-00340) and sdhE (APA01_11050) from Acetobacter pasteurianus DSM3509 into the gdhS locus (GOX2015 / GOX_RS11350) | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769949 |
IK002.1 | ΔuppΔgdhS::sdhCDABEΔpdc::ndh IK001 derivative with genomically integrated ndh from G. oxydans DSM3504 (GLS_c05650) into the pdc locus (GOX1081 / GOX_RS06555) | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769950 |
IK002.1* | ΔuppΔgdhS::sdhCDABEΔpdc::ndh IK001 derivative with genomically integrated ndh from G. oxydans DSM3504 (GLS_c05650) into the pdc locus (GOX1081 / GOX_RS06555). After 24 h or 28 h of growth under controlled conditions in a DASGIP bioreactor. | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769951 |
IK003.1 | ΔuppΔgdhS::sdhCDABEΔpdc::ndhΔgdhM::sucCD IK002.1 derivative with genomically integrated sucCD (GDI_2951-2952) from Gluconacetobacter diazotrophicus DSM5601 into the gdhM locus (GOX0265 / GOX_RS02475) | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769952 |
IK003.1 | ΔuppΔgdhS::sdhCDABEΔpdc::ndhΔgdhM::sucCD IK002.1 derivative with genomically integrated sucCD (GDI_2951-2952) from Gluconacetobacter diazotrophicus DSM5601 into the gdhM locus (GOX0265 / GOX_RS02475) | Oxford Nanopore MinION | R9 2D protocol | ERR1769944 |
IK003.1* | ΔuppΔgdhS::sdhCDABEΔpdc::ndhΔgdhM::sucCD IK002.1 derivative with genomically integrated sucCD (GDI_2951-2952) from Gluconacetobacter diazotrophicus DSM5601 into the gdhM locus (GOX0265 / GOX_RS02475). After 24 h or 28 h of growth under controlled conditions in a DASGIP bioreactor. | Illumina MiSeq | 2x150bp paired-end TruSeq PCR-free | ERR1769953 |
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